Use of Fatty Acid Methyl Ester Profiles for Discrimination of Bacillus

Use of Fatty Acid Methyl Ester Profiles for Discrimination of Bacillus

  • use of fatty acid methyl ester profiles for discrimination

    Use of Fatty Acid Methyl Ester Profiles for Discrimination

    The goal of this study was to determine if cellular fatty acid methyl ester (FAME) profiling could be used to distinguish among spore samples from a single species ( Bacillus cereus T strain) that were prepared on 10 different medium formulations. To analyze profile differences and identify FAME biomarkers diagnostic for the chemical constituents in each sporulation medium, a

  • use of fatty acid methyl ester profiles for discrimination

    Use of Fatty Acid Methyl Ester Profiles for Discrimination

    The goal of this study was to determine if cellular fatty acid methyl ester (FAME) profiling could be used to distinguish among spore samples from a single species (Bacillus cereus T strain) that were prepared on 10 different medium formulations.

  • use of fatty acid methyl ester profiles for discrimination

    Use of Fatty Acid Methyl Ester Profiles for Discrimination

    Use of Fatty Acid Methyl Ester Profiles for Discrimination of Bacillus cereus T-Strain Spores Grown on Different Media Article (PDF Available) in Applied and Environmental Microbiology 76(6):1902

  • use of fatty acid methyl ester profiles for discrimination

    Use of Fatty Acid Methyl Ester Profiles for Discrimination

    Use of Fatty Acid Methyl Ester Profiles for Discrimination of Bacillus cereus T-Strain Spores Grown on Different Media By Christopher J. Ehrhardt, Vivian Chu, TeeCie Brown, Terrie L. Simmons, Brandon K. Swan, Jason Bannan and James M. Robertson

  • determination of antibiotic susceptibility and fatty acid

    Determination of antibiotic susceptibility and fatty acid

    Request PDF on ResearchGate | Determination of antibiotic susceptibility and fatty acid methyl ester profiles of Bacillus cereus strains isolated from different food sources in Turkey | In this

  • fatty acid methyl ester (fame) analysis of moderately

    Fatty Acid Methyl Ester (FAME) Analysis of Moderately

    Fatty acid methyl ester (FAME) analysis of the bacterial isolates was done to identify the bacterial strain and analyse the Base Wash: Sodium hydroxide (10.8 g) (certified ACS) was fatty acid profile

  • fatty acid profiles for differentiating growth medium

    Fatty Acid Profiles for Differentiating Growth Medium

    Fatty Acid Profiles for Differentiating Growth Medium Formulations Used to Culture Bacillus cereus T-strain Spores* Article in Journal of Forensic Sciences 60(4) · April 2015 with 50 Reads

  • fatty acid profiles for differentiating growth medium

    Fatty Acid Profiles for Differentiating Growth Medium

    Bacillus cereus T‐strain spores were grown in medium formulations supplemented with one of the following: peptone (meat protein), tryptone (casein protein), soy protein, and brain–heart infusion. Cellular biomass was profiled with fatty acid methyl ester (FAME) analysis.

  • from learning taxonomies to phylogenetic learning

    From learning taxonomies to phylogenetic learning

    Machine learning techniques have shown to improve bacterial species classification based on fatty acid methyl ester (FAME) data. Nonetheless, FAME analysis has a limited resolution for discrimination of bacteria at the species level. In this paper, we approach the species classification problem from a taxonomic point of view.

  • fatty acid ethyl ester - an overview | sciencedirect topics

    Fatty Acid Ethyl Ester - an overview | ScienceDirect Topics

    Fatty Acid Ethyl Ester. Fatty acid ethyl esters (FAEE) are formed by an enzyme-mediate esterification of ethanol with fatty acids or fatty acyl-coenzyme A. It has been shown that FAEE and the FAEE synthase are predominantly present in those organs most often damaged by ethanol abuse, notably the pancreas and liver.

  • identification of microorganisms using fatty

    IDENTIFICATION OF MICROORGANISMS USING FATTY

    IDENTIFICATION OF MICROORGANISMS USING FATTY ACID METHYL ESTER (FAME) ANALYSIS AND THE MIDI SHERLOCK® MICROBIAL IDENTIFICATION SYSTEM Craig Kunitsky, Gerard Osterhout, and Myron Sasser MIDI, Inc. Newark, DE, USA INTRODUCTION For more than 15 years, a substantial portion of the pharmaceutical industry has

  • differentiation of bacillus endospore species from fatty

    Differentiation of Bacillus endospore species from fatty

    In the Bacillus species, some fatty acids such as i15:0, a15:0, i17:0, and a17:0 have especially high intensities that can be used effectively for differentiation from non-Bacillus species. In other words, these fatty acids, similar to DPAME, can be used for detection of Bacillus species.

  • microbiology society journals | bacillus nealsonii sp. nov

    Microbiology Society Journals | Bacillus nealsonii sp. nov

    One of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus Bacillus, is described on the basis of phenotypic characterization, 16S rDNA sequence analysis and DNA–DNA hybridization studies. It is a Gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. The spores of this novel bacterial species exhibited resistance to UV, γ

  • genotypic and phenotypic analysis of tterrnicin a

    Genotypic and phenotypic analysis of tterrnicin A

    The fatty acid composition of 20 strains known to be zwittermicin A producers and 20 strains known to be non-producers was determined. Cluster analysis of the fatty acid methyl ester (FAME) profiles revealed that zwittermicin A producers grouped together in two clusters, apart from most non-producers. Discriminant analysis of the FAME profiles

  • the use of fatty acid methyl ester analysis (fame) for the

    The use of fatty acid methyl ester analysis (FAME) for the

    Four hundred twenty‐eight bacterial strains were isolated from which 385 were characterized by fatty acid methyl ester analysis (FAME). The isolates were grouped into 41 clusters on the basis of their FAME profiles, 20 isolates remained ungrouped.

  • lipids - wiley online library

    Lipids - Wiley Online Library

    The fatty acids and sterols of ungerminated chlamydospores of the vesicular‐arbuscular (VA) endophyteAcaulospora laevis were examined by gas chromatography and mass spectrometry. The total lipid content of the spores was 45.5% of the spore dry weight. Predominant fatty acids were palmitoleic (52.5%), palmitic (25.5%) and oleic (7.4%).

  • use of fatty acid methyl esters as biomarkers to determine

    use of fatty acid methyl esters as biomarkers to determine

    The use of fatty acid methyl esters (FAME) as biomarkers to identify groups of microorganisms was studied. A database was constructed using previously published results that identify FAME biomarkers for aerobic, anaerobic and facultatively aerobic bacteria.

  • identification of fatty acids in bacillus cereus | protocol

    Identification of Fatty Acids in Bacillus cereus | Protocol

    Table 1: Identification of fatty acids from Bacillus cereus ATCC 14579. Retention times, equivalent chain length (ECL) values, and molecular ions for fatty acid methyl esters (FAMEs) are shown, together with molecular ions and diagnostic ions for DMOX and picolinyl derivatives.

  • gc/fid analysis of fatty acid methyl esters without

    GC/FID Analysis of Fatty Acid Methyl Esters without

    The fatty acids are esterified to form fatty acid methyl ester (FAME) analogues, which are volatile, usually nonreactive and separate well in GC columns. The composition analysis involves (1) the identification of the FAME and (2) the quantification of its concentration in the mixture.

  • use of fatty acid methyl ester profiles to compare copper

    Use of fatty acid methyl ester profiles to compare copper

    Use of fatty acid methyl ester profiles to compare copper-tolerant and copper-sensitive strains of Pantoea ananatis. Phytopathology 97: 1298-1304 This document is currently not available here.

  • rapid pyrosequencing and fatty acid analysis for

    Rapid Pyrosequencing and Fatty Acid Analysis for

    All 30 presumptive Bacillus cereus isolates were identified as B. cereus.Atleasttwose- Fatty acid methyl ester profiles have long been recognized as useful biochemical markers for bacterial classification and characterization. The analysis of fatty acids

  • microalgal species selection for biodiesel production

    Microalgal Species Selection for Biodiesel Production

    predict key fuel properties from fatty acid profiles. For example, FAME composition was used to calculate CN [14] whereas other researchers used iodine—and saponification values to calculate the CN [15]. The Smittenberg relation was used to estimate the density of saturated methyl esters

  • catabolic repression of bacterial sporulation

    Catabolic repression of bacterial sporulation

    Use of Fatty Acid Methyl Ester Profiles for Discrimination of Bacillus cereus T-Strain Spores Grown on Different Media Functional Analysis of the Stability Determinant AlfB of pBET131, a Miniplasmid Derivative of Bacillus subtilis (natto) Plasmid pLS32

  • miniature sensors for biological warfare agents using

    Miniature Sensors for Biological Warfare Agents using

    Issued by Sandia National Laboratories, operated for the United States Department of Energy by Sandia Corporation. NOTICE: This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government, nor any agency thereof, nor any of

  • rapid pyrosequencing and fatty acid analysis for

    Rapid Pyrosequencing and Fatty Acid Analysis for

    All 30 presumptive Bacillus cereus isolates were identified as B. cereus.Atleasttwose- Fatty acid methyl ester profiles have long been recognized as useful biochemical markers for bacterial classification and characterization. The analysis of fatty acids

  • identification of three strains of mycobacterium species

    Identification of Three Strains of Mycobacterium Species

    The cellular fatty acid profiles of 67 strains belonging to three different species of the genus Mycobacterium were determined by gas chromatography of the fatty acid methyl esters, using the MIDI Sherlock® Microbial Identification System (MIS).

  • analysis of chemical signatures of alkaliphiles using

    Analysis of Chemical Signatures of Alkaliphiles using

    Fatty acid methyl ester analysis diverse chemical and physical properties determine the variety of their biochemical functions. This diversity, which is found in unique combinations in various bacterial species, makes fatty acid profilinga useful identificationtool. The cellular fatty acid analysis for bacterial

  • fatty acid methyl ester

    Fatty acid methyl ester

    Fatty acid methyl esters (FAME) are a type of fatty acid ester that are derived by transesterification of fats with methanol. The molecules in biodiesel are primarily FAMEs, usually obtained from vegetable oils by transesterification. They are used to produce detergents and biodiesel.

  • differentiation of bacillus endospores from gas

    Differentiation of Bacillus Endospores from Gas

    and used for differentiation include dipicolinic acid methyl ester (DPAME), fatty acid methyl esters (FAMEs), 3-methyl-2-butenoic acid methyl ester (3M2BAME), 2-butenoic acid methyl ester (2BAME), and several methylated sugars. TCM of endospores was performed based on hydrolysis and methylation at

  • fame (fatty acid methyl esters) market - global industry

    FAME (Fatty Acid Methyl Esters) Market - Global Industry

    Fatty acid methyl esters (FAME) are produced by reacting fatty acids and methanol. They are also present in biodiesels and are similar to those in conventional diesel but expel lower concentrations of toxic emissions and thus, are non-toxic and biodegradable. They are produced from vegetable oils and animal fats by the process of

  • microalgal species selection for biodiesel production

    Microalgal Species Selection for Biodiesel Production

    energy cost) [3]. In contrast, transesterification-derived regular biodiesel, where fatty acids are converted to fatty acid methyl esters (FAMEs), is a conversion technology that can be economically applied at remote biomass production facilities for servicing production site and community energy and transport fuel demands today.

  • domain swapping localizes the structural determinants of

    Domain Swapping Localizes the Structural Determinants of

    For GC/MS analysis, fatty acid methyl esters of interest were partially purified from the total fatty acid methyl ester fraction by HPLC using an Agilent 1100 Series HPLC system with the fraction collector collecting 0.5-ml fractions from 2 × 12.5-cm Whatman Partisphere C 18 columns connected in series and using a linear solvent gradient